Sterile intra-amniotic inflammation appears to be more common than microbial-associated intra-amniotic inflammation in patients with preterm labor and intact chorioamniotic meranes . This process is also frequently observed in patients with a sonographic short cervix [ 21 ] and in those with preterm prelabor rupture of the meranes (PPROM) with clinical chorioamnionitis at term [ 22 ].
The culture pellet was suspended in sterile paddy field water (pH 6.8; filter sterilized with a 0.22-μm filter, Millex-GV) at an optimum cell density of 2 × 10 7 cells/mL. Glass slides (76 × 26 mm, Hi-Media, India) were incubated by submerging half of the slide into a bacterial suspension for different time intervals (1, 6, 16, 24, 40, 48, 64, 72, 160 and 190 h) at 28°C.
Stock solutions were either made up on the day of the experiment or taken from aliquots stored at -80 C for no longer than 1 week prior to use. After 0.22 μm filtration with Millex GV filter units (Millipore, Ireland), stock solutions were diluted to the corresponding
The soluble LC was sterile-filtered using a 0.22-µm polyvinylidene fluoride 25-mm Millex-GV syringe-driven filter unit (Millipore, Bedford, MA, USA). SDS-PAGE analyses confirmed that the protein was >90% pure, and protein concentration was determined by the MicroBCA assay.
2200^. Sterile filtered buffer (Millex-GV, Milli pore, France) and disposable equipment were used. After centrifugation the clear supernatant was collected and the protease activity was measured with a modifiion of the sensitive end point assay for airborne
24/11/2020 Automated production of [18F]MK-6240 was implemented for the first time in the CFN-MPS200 module. Three consecutive productions of [18F]MK-6240 compli…
The wells of a 96‐well plate (Corning‐Costar, Sigma‐Aldrich) were filled with 200 μl of broth (selected depending on the agar from which the bacteria were isolated) and one colony of each strain to be tested was suspended in the wells with a sterile loop.
[Show full abstract] examined adsorptive losses of electrolytically-labelled, biologically-active bPTH 1-34 onto sterile filtration devices (Millex GV, Millipore Corp.) in solutions of 0.1 M
into 2-L Corning sterile roller bottles (431133) and stored at room temperature. RNase A solutions were ﬁltered through 220-nm Millipore Millex-GV syringe ﬁlters (SLGVR25LS) before use. The concentration of RNase A photometer at 280 nm, using an extinction
1/1/2016 Virus was eluted as a discreet peak (SP Peak, 40% buffer B) in a final formulation of 22 mmol/l sodium citrate, 13.1 mmol/l citric acid and 200 mmol/l sodium chloride, at ∼pH 4.8 The sample was then sterile filtered with Millex-GV syringe filter, 0.22 µmol/l (EMD
ilized by the use of a 0.22- m-pore-size syringe ﬁlter (Millex-GV;Milli-pore). Staurosporine stock solution (1.0 mM) was prepared in sterile di-methyl sulfoxide (DMSO) (American Type Culture
pushed through sterile Millex-GV PVDF 0.45 µm fil-ters using 3 ml sterile Luer-Lok plastic syringes. Sam-ples were then split into two 1 ml samples, for qPCR analysis and cell culture. The
To ensure you always have the most accurate information for using Millipore ® products, we have compiled several published solubility and compatibility tables to provide a comprehensive compatibility guide for Millipore ® meranes, filtration devices, ultrafiltration products, centrifugal filters, and MultiScreen ® filter plates.
The soluble LC was sterile-filtered using a 0.22-µm polyvinylidene fluoride 25-mm Millex-GV syringe-driven filter unit (Millipore, Bedford, MA, USA). SDS-PAGE analyses confirmed that the …
Antibodies were further purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare) run in PBS and pooled fractions were sterile filtered using millex–GV filter units (Millex).
carbohydrates, which was added to the cooled broth after sterile filtration (Millex-GV, 0.22 um, Millipore). All bacterial cultures used in the adhesion experiment were grown for 48h
Sterile Filtration Good cell culture practices start with proven products like Stericup® and Millex® filters. Reagents, Chemicals and Labware Cleaning of Lab Equipment Inorganic Reagents Lab Filtration Organic Synthesis Novabiochem Peptide and DNA Solvents
In the first passage this medium was supplemented with 1.5% (w/v) filter‐sterilized (Millex‐GV, 0.22 µm, Millipore, Billerica, MA) lactose as the sole carbohydrate source. The resulting cultures were then used as a 1% inoculum for a second passage where the medium was supplemented with 1.6% (w/v) sterile‐filtered HMO ( Fig. 1, b ).